| JAK3

Janus Kinase 3 (JAK3) is a tyrosine kinase encoded by the JAK3 gene and belongs to the janus kinase family. JAK3 is required for signaling of the type I receptors that use the common gamma chain (γc).Studies suggest Jak3 plays essential roles in immune and nonimmune cell physiology. Epithelial Jak3 is important for the regulation of epithelial-mesenchymal transition, cell survival, cell growth, development, and differentiation. Growth factors and cytokines produced by the cells of hematopoietic origin use Jak kinases for signal transduction in both immune and nonimmune cells.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.15	0
OD	2.15	1.46	0.87	0.6	0.39	0.28	0.21	0.09
Corrected OD	2.06	1.37	0.78	0.51	0.3	0.19	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human JAK3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human JAK3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	87-96
	Average (%)	91	92
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98